The United States and the European Union imposed sanctions on top officials in Myanmar who are linked to last month's military coup, as anti-coup protesters took to the streets again, despite the regime’s violent and increasingly deadly pushback.

The European Union issued a freeze on assets and a visa ban for the military’s commander-in-chief, Min Aung Hlaing, as well as nine other senior military officers and the head of the country’s election commission.

An EU statement said the sanctions are part of the bloc’s “robust response to the illegitimate overthrowing of the democratically elected government and the brutal repression by the junta against peaceful protesters.”

A large image that has an X mark on the face of Commander in chief Senior Gen. Min Aung Hlaing, also chairman of the State Administrative Council, lies on a road as anti-coup protesters gather outside the Hledan Centre in Yangon, Myanmar, Feb. 14, 2021. — FILE - A image that has an X mark on the face of Commander in chief Senior Gen. Min Aung Hlaing is seen on a road as anti-coup protesters gather outside the Hledan Centre in Yangon, Myanmar, Feb. 14, 2021.

Meanwhile, the U.S. Treasury Department sanctioned Myanmar's police chief and an army special operations commander Monday, saying they were responsible for using lethal force against demonstrators. The United States has already placed sanctions on Myanmar's top coup leaders.

There were no reports of an immediate response from the junta to the growing list of punitive actions taken by the international community.

“The Burmese security forces’ lethal violence against peaceful protesters must end," said Andrea Gacki, director of the U.S. Treasury's Office of Foreign Assets Control.

The sanctions came as hundreds of Myanmar demonstrators marched before dawn Monday along a main road in Mandalay, many of them doctors, nurses, students and other medical personnel wearing white coats, repeating a similar demonstration carried out the day before.

Medical staff and students take part in an early morning protest against the military coup and crackdown by security forces on… — FILE - Medical staff and students take part in a protest against the military coup and crackdown by security forces on demonstrations in Mandalay, March 21, 2021.

Scores of motorists in the commercial capital of Yangon honked their horns in response to a call on social media, according to Reuters.

Myanmar has been in turmoil ever since the military jailed de facto leader Aung San Suu Kyi and other members of the civilian government on February 1. At least three people were killed Sunday in violent clashes between police and civilians, including two in Mandalay, Myanmar’s second-largest city and a major hub of opposition.

The U.N. Human Rights Office said Monday that at least 224 civilians have been confirmed killed at the hands of security forces, including while in custody. The United Nations said the real figure could be more than 250, according to spokesman Farhan Haq.

An activist group, the Assistance Association for Political Prisoners, said more than 2,000 people have been detained since the coup began.

Australian authorities confirmed Sunday that two of its citizens identified as businesspeople were detained in Myanmar. Australia’s government declined to provide further details, citing the privacy of the concerned parties.

On Friday, United Nations Secretary-General Antonio Guterres strongly condemned the continuing brutality of the Myanmar military after at least eight anti-coup demonstrators were killed in the central town of Aungban in eastern Shan state.

FILE - U.N. Secretary-General Antonio Guterres.

“The killing of peaceful demonstrators and arbitrary arrests, including of journalists, is utterly unacceptable,” Guterres’ spokesman Stephane Dujarric told reporters Friday. “The military continues to defy calls, including by the (U.N.) Security Council, to end violations of fundamental human rights and return to the path of democracy.”

He said that a firm, unified international response is urgently needed.

“The secretary-general will continue to stand with the people and their aspirations to achieve a peaceful, stable and prosperous Myanmar,” Dujarric added. (VOA)

•PHOTO: Motorists pass a burning barricade during a crackdown by security forces on demonstrations by protesters against the military coup in Mandalay, March 22, 2021, in this photo taken and received courtesy of an anonymous source.

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